Rat anti-Mouse IgM (Chain mu) Antibody (FITC)

Catalog No. ABIN118420

Quick Overview for Rat anti-Mouse IgM (Chain mu) Antibody (FITC) (ABIN118420)

Target: IgM

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LO-MM-9

Isotype: IgG2a

Product Details

Binding Specificity: Chain mu

Reactivity: Mouse

Host: Rat

Purification: Affinity chromatography on mouse IgM/Sepharose 4B

Immunogen: Polyclonal and monoclonal mouse IgM. Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.

Application Details

Application Notes: Flow Cytometry: 5 μg/mL, use 5 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgM

Target Type: Antibody

Background: Synonyms: Immunoglobulin M mouse